Prashant Singh, Ph.D.

Assistant Professor

 psingh2@fsu.edu    850.644.1829    Vita

Prashant Singh

Get to know Dr. Singh

Interests

Dr. Singh is a food safety microbiologist by training, and his core area of research focus is the development of rapid methods for the detection of foodborne pathogens. His laboratory focuses explicitly on developing methods for the specific detection of virulent strains of Shiga toxin-producing non-O157 Escherichia coli and Salmonella. His second area of research interest is the development of rapid methods for seafood species identification.

Education

  • Ph.D., University of Missouri
  • Graduate Certificate, Food Safety & Defense, University of Missouri
  • M.S., Vellore Institute of Technology
  • B.S., Delhi University

Publications and Other Scholarly Activities

Velez, F. J., Bosilevac, J., & Singh, P. (2021). Validation of High-Resolution Melting Assays for the Detection of Virulent Strains of Escherichia coli O26 and O111 in Beef and Pork Enrichment Broths. Food Control. 128, 108123.

Sharma, L., Nagpal, R., Jackson, C. R., Patel, D., & Singh, P. (2021). Antibiotic-resistant bacteria and gut microbiome communities associated with wild-caught shrimp from the United States versus imported farm-raised retail shrimp. Scientific reports, 11(1), 1-14.

Indugu, N., Sharma, L., Jackson, C. R., & Singh, P. (2020). Whole-Genome Sequence Analysis of Multidrug-Resistant Enterobacter hormaechei Isolated from Imported Retail Shrimp. Microbiology Resource Announcements, 9(50).

Sharma, L., Watts, E., & Singh, P. (2020). High-resolution real-time PCR melting curve assay for identification of top five Penaeidae shrimp species. LWT Food Science and Technology, 109983.

Singh, P., Cubillos, G., Kirshteyn G., Bosilevac, JM. (2020). High-resolution melting real-time PCR assays for detection of Escherichia coli O26 and O111 strains possessing Shiga toxin genes. LWT Food Science and Technology. 131, 109785.

Sangokunle, OO., Sathe, SK., Singh, P. (2020). Purified starches from 18 pulses have markedly different functionality. Starch.72(11-12), 2000022.

Rather, I. A., Bajpai, V. K., Ching, L. L., Majumder, R., Nam, G. J., Indugu, N., Singh, P … & Kamli, M. R. (2020). Effect of a bioactive product SEL001 from Lactobacillus sakei probio65 on gut microbiota and its anti-colitis effects in a TNBS-induced colitis mouse model. Saudi Journal of Biological Sciences, 27(1), 261-270.

Singh, P., Liu, Y., Bosilevac, JM., Mustapha, A. (2018). Detection of shiga toxin producing Escherichia coli, stx1, stx2 and Salmonella by high resolution melt curve multiplex real-time PCR. Food Control 96, 251-259.

Tian, K., Chen, X., Luan, B., Singh, P., Yang, Z., Gates, K. S., … & Gu, L. Q. (2018). Single locked nucleic acid-enhanced nanopore genetic discrimination of pathogenic serotypes and cancer driver mutations. ACS Nano, 12(5), 4194-4205.

Singh, P., Hung, Y. C., & Qi, H. (2018). Efficacy of peracetic acid in inactivating foodborne pathogens on fresh produce surface. Journal of food science, 83(2), 432-439.

Liu, Y., Singh, P., & Mustapha, A. (2018). Multiplex high-resolution melt-curve real-time PCR assay for reliable detection of Salmonella. Food Control, 91, 225-230.

Liu, Y., Singh, P., & Mustapha, A. (2018). High-resolution melt curve PCR assay for specific detection of Escherichia coli O157: H7 in beef. Food Control, 86, 275-282.

Ouf, J.M.M., Yuan Y., Singh, P., Mustapha, A. (2017). Detection of viable but nonculturable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR. International Journal of Agricultural Science and Food Technology 3(2), 026-031.

Singh, P., Pfeifer, Y., Mustapha, A. (2016). Multiplex real-time PCR assay for the detection of extended-spectrum β-lactamase (ESBL) and carbapenemase genes using melting curve analysis. Journal of Microbiological Methods, 124, 72-78.

Forghani, F., Singh, P., & Oh, D. H. (2015). A novel pentaplex real time (RT)-PCR high resolution melt curve assay for simultaneous detection of emetic and enterotoxin producing Bacillus cereus in food. Food Control, 60, 560-568.

Singh, P., & Mustapha, A. (2015). Multiplex real-time PCR assays for detection of eight Shiga toxin-producing Escherichia coli in food samples by melting curve analysis. International Journal of Food Microbiology, 215, 101-108.

Pophaly, S. D., Poonam., Singh, P., Kumar, H., Tomar, S. K., Singh, R. (2014). Selenium enrichment of lactic acid bacteria and Bifidobacteria: A functional food perspective. Trends in Food Science and Technology, 39(2), 135-145.

Kaliyaperumal, A., Nanda, D. K., UmaMaheswari, T., Thiagaraja, H., Singh, P., Singh, R. (2014). Assessment of expression of Leloir pathway genes in wild type galactose fermenting Streptococcus thermophilus by real time PCR. European Food Research and Technology, 239(5), 895-903.

Blumhagen, A., Singh, P., Mustapha, A., Yu, Q. (2014). Plasma deactivation of oral bacteria seeded on hydroxyapatite disks as tooth enamel analogue. American Journal of Dentistry, 27(2), 84-90.

Singh, P., & Mustapha, A. (2014). Development of a real-time PCR melt curve assay for simultaneous detection of virulent and antibiotic resistant Salmonella. Food Microbiology, 44,6-14.

Kaliyaperumal, A., UmaMaheswari, T., Thiagaraja, H., Nanda, D. K., Singh, P., Singh, R. (2014). Preparation of low galactose yogurt using cultures of Gal+ Streptococcus thermophilus in combination with Lactobacillus delbrueckii ssp. bulgaricus. Journal of Food Science and Technology, 51(9), 2183-2189.

UmaMaheswari, T., Singh, R., Singh, P., Tomar, S. K. (2014). Polyphasic characterization, phylogenetic analysis and technological properties of Streptococcus thermophilus strains isolated from plant sources. International Journal of Dairy Technology, 67(1), 117-122

Singh, P., & Mustapha, A. (2013). Multiplex TaqMan detection of pathogenic and multi-drug resistant Salmonella. International Journal of Food Microbiology, 166(2), 213-218.

Anbukkarasi, K., UmaMaheswari, T., Hemalatha, T., Nanda, D., Singh, P., Rashmi, H. M., & Singh, R. (2013). Production of low browning mozzarella cheese: Screening and characterization of wild galactose fermenting Streptococcus thermophilus strains. International Journal of Advanced Research, 1, 83-96.

Iyer, R., Tomar, S. K., Mohanty, A. K., Singh, P., Singh, R. (2011). Bioprospecting Streptococcus thermophilus strains from Indian fermented milk products for folate production. Dairy Science & Technology 91(2), 237-246.

Nanda, D. K., Tomar, S. K., Singh, R., Mal, G., Singh, P., Arora, D. K., Joshi, B. K., Kumar, D. (2011). Phenotypic and genotypic characterization of Lactobacilli isolated from camel cheese produced in India. International Journal of Dairy Technology, 64(3), 437-443.

Jain, A., Gour, D. S., Bisen, P. S., Prashant., Dubey, P. P., Sharma, D. K., Joshi, B. K., Kumar, D. (2009). Single nucleotide polymorphism in alpha-lactalbumin 1 gene of Jamunapari goat. Small Ruminant Research, 82, 156-160.
Prashant., Tomar, S. K., Singh, R., Gupta, S. C., Joshi, B. K., Arora, D. K., Kumar, D. (2009). Phenotypic and Genotypic characterization of Lactobacillus from Churpi Cheese. Dairy Science and Technology, 89, 531-540.
Jain A., Gour D. S., Dubey P. P., Prashant, Bisen P. S., Kumar D. (2008). Single strand confirmation polymorphism (SSCP) detection in alpha-lactalbumin Gene of Indian Jakhrana milk goats. Acta Agri Scand A Animal Science, 58, 205-208.Prashant, Gour D. S., Dubey P. P., Jain A., … & Kumar, D. (2008). Sex determination in 6 bovid species by duplex PCR. Journal of Applied Genetics, 49(4), 379-381.

Book chapter:

Singh, P., Velez, F., & Forghani, F. (2021). Methods for Multiplex Real-Time PCR Melting Curve Assays for Pathogen Detection. In Irshad M. Sulaiman, PhD (Ed.), Diagnosis of Pathogenic Microorganisms Causing Infectious Diseases (pp. 16). CRC Press.

Zaffran, V., Kirshteyn, G., Singh, P. (2019). Application of Predatory Bacteria for Enhancing Food Safety and Mitigating Clinical Pathogens. In: Application of Predatory Bacteria for Enhancing Food Safety. Editors: Santosh Mishra. Apple Academic Press

Singh, P., Gao, F., Bernat, A. (2019). Nanobodies and their in vivo applications. In: Advanced Biosensors for Health Care Applications. Editors: Inamuddin, Raju Khan, Ali Mohammad and Abdullah M. Asiri. Elsevier. ISBN: 9780128157435

Mustapha, A., & Singh, P. (2013). Applications of molecular-based foodborne pathogen detection. In: Microbial Food Safety and Preservation Techniques. Editors: V Ravishankar Rai and A Jamuna Bai. CRC Press/Taylor & Francis Group ISBN: 9781466593060

Accomplishments

  • Patent No. 20160032366: Rapid assay for detection of shiga toxin producing E. coli and antibiotic resistant pathogens.
  • IFT Feeding Tomorrow graduate scholarship recipient
  • Faye W. Furguson scholarship recipient
  • Italian government scholarships recipient
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